The Manual for trainers aims to facilitate the conduction of the UniCulture training course in the most appropriate and effective manner, with guidelines on the training process, participants’ assessment, and feedback collection. However, it also allows the trainer enough freedom to customize the content and apply selected approaches according to the specific training group dynamics and participants. The Manual consists of five chapters: (i) Training in diverse and intercultural environments, (ii) Training of trainers, (iii) UniCulture training design, (iv) Evaluation, feedback, and follow-up and (v) Using the UniCulture Digital Library. Readers are encouraged to use both complementary materials (Handbook and Workbook) available on the project website if they decide for self-training, to provide feedback on their progress or any query using the contact details on the website.